PTEN-P1 Antibody

SKU: orb345623

Description

PTEN-P1 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:160,000, WB: 1:500 to 1:2,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 55 kDa in size corresponding to PTEN-P1 protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to amino acids near the N-terminal end of human PTEN-P1 protein.
• Purity: This affinity-purified antibody is directed against human PTEN protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against human PTEN protein. A BLAST analysis was used to suggest cross reactivity with PTEN proteins from mouse, dog sources based on 82% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.15 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-PTEN-P1 antibody, Phosphatase and tensin homolog 2, Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, PTEN, MMAC1, TEP1

Western blot using Biorbyt's affinity purified anti-PTEN-P1 antibody shows detection at ~55kDa (arrowhead) of endogenous PTEN-P1 in whole cell lysates from human derived cell lines HeLa (p/n orb348668) [lane 4], HEK293 (p/n orb348669) [lane 5] and MCF7 (p/n orb348664) [lane 6]. Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide. The identity of lower molecular weight bands in lane 4 is unknown. Briefly, each lane contains approximately 35 µg of lysate. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:10000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).

Quick Database Links

UniProt

O43460

RefSeq:73765544