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    PTEN antibody

    Catalog Number: orb318864

    DispatchUsually dispatched within 5-10 working days
    $ 337.00
    Catalog Numberorb318864
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to PTEN
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Mouse, Porcine, Primate, Rat
    ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PTEN. The exact sequence is proprietary.
    Dilution rangeWB: 1:500-1:1000, IHC-P: 1:100-1:200, IF/ICC: 1:100-1:500
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetPTEN
    Entrez19211, 5728
    UniProt IDO08586, P60484
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesanti MMAC1 antibody, anti TEP1 antibody, anti Phos
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    PTEN antibody

    Western blot analysis of PTEN expression in mouse brain (A), rat brain (B) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 54 kD)

    PTEN antibody

    Immunohistochemical analysis of PTEN staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    PTEN antibody

    Immunofluorescent analysis of PTEN staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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