PTCH1 antibody

• Catalog Number: orb107648
• Category: Antibodies
• Description: Goat polyclonal antibody to PTCH1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 161; 161; 154; 144
• Target: PTCH1 (aa266-280)
• Entrez: 5727
• Protein Sequence: EELKKINYQVDSWEE
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PTCH1 antibody, anti patched 1 antibody, anti
• Note: For research use only
• Application notes: WB: Approx 140kDa band observed in Mouse fetal Kidney lysates (calculated MW of 159kDa according to NP_032983.1). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 8µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 5µg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Mouse fetal Kidney lysate (35ug protein in RIPA buffer) using PTCH1 antibody

Immunofluorescence analysis of HeLa cells using PTCH1 antibody