PTCH1 antibody

• Catalog Number: orb125007
• Category: Antibodies
• Description: Goat polyclonal antibody to PTCH1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:16000, IHC-P: 8 μg/ml
• Conjugation: Unconjugated
• MW: 161; 161; 154; 144
• Target: PTCH (C Terminus)
• Entrez: 5727
• Protein Sequence: ELQDVECEERPR
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PTCH antibody, anti patched homolog (Drosophi
• Note: For research use only
• Application notes: IHC: Paraffin embedded Human Brain (Coretx). Recommended concentration: 8µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the Golgi of HeLa and NIH3T3 cells. Recommended concentration: 10µg/ml.
• Expiration Date: 12 months from date of receipt.

orb125007 (3.75µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunofluorescence analysis of HeLa cells using PTCH1 antibody

orb125007 (3.8µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunofluorescence analysis of NIH3T3 cells using PTCH1 antibody