PTBP2 antibody

• Catalog Number: orb19658
• Category: Antibodies
• Description: Goat polyclonal antibody to PTBP2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Dilution range: ELISA: 1:1000, WB: 2-3μg/ml, IF/ICC: 10μg/ml
• Conjugation: Unconjugated
• MW: 58.1; 58.6; 59.6; 58.0; 57.6; 57.5
• Target: PTBP2
• Entrez: 58155
• Protein Sequence: SKKFKGEDKMD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PTBP2 antibody, anti polypyrimidine tract bin
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Preliminary experiments gave an approx 35kDa band in lysates of cell line NIH3T3 after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.5kDa according to NP_067013.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of paraformaldehyde fixed A431 cells using PTBP2 antibody.

Western blot analysis of Mouse Testes (A) lysate and nuclear NIH3T3 (B) cell lysate using PTBP2 antibody.