PSME1 antibody

• Catalog Number: orb340901
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PSME1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Immunogen: Recombinant full length protein of human PA28 alpha
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PSME1
• Entrez: 19186, 5720
• UniProt ID: P97371, Q06323
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: IFI5111; Proteasome activator complex subunit 1; 1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PA28 alpha expression in MCF7 (A), BT474 (B), mouse spleen (C), mouse liver (D) whole cell lysates. (Predicted band size: 26; 28 kD; Observed band size: 28 kD)

Immunofluorescent analysis of PA28 alpha staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.