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Catalog Number | orb20247 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to PSMB8 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:8000, WB: 0.01-0.03 μg/ml, IHC-P: 3.75 μg/ml |
Conjugation | Unconjugated |
MW | 29.8; 30.4 |
Target | LMP7 |
Entrez | 5696 |
Protein Sequence | DVSDLLHQYREANQ |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti PSMB8 antibody, anti proteasome (prosome, mac Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 22kDa band observed in Human Liver, Lung and Lymph Node lysates (calculated MW of 29.8kDa according to precursor NP_004150.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schwarz et al, J Immunol. 2000 Jul 15;165(2):768-78. PMID: 10878350. Recommended concentration: 0.01-0.03 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb20247 (0.01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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