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Catalog Number | orb1249051 |
---|---|
Category | Antibodies |
Description | PSMB4 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Bovine, Canine |
Reactivity | Human, Mouse, Rat |
Immunogen | The immunogen for this antibody is: C-NRFQIATVTE |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 25-26kDakDa band observed in lysates of cell lines HEK293, HeLa, and K562 and in Mouse and Rat Liver lysates (calculated MW of 29.1kDa according to NP_002787.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | PSMB4 |
UniProt ID | P28070 |
NCBI | NP_002787.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | PSMB4, proteasome (prosome, macropain) subunit, be Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 25-26kDakDa band observed in lysates of cell lines HEK293, HeLa, and K562 and in Mouse and Rat Liver lysates (calculated MW of 29.1kDa according to NP_002787.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1249051 (0.1 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1249051 (0.3 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA, FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Bovine, Canine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Canine, Rat | |
Human, Mouse | |
Goat | |
Polyclonal | |
Unconjugated |
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