PSMB4 Antibody

• Catalog Number: orb1248879
• Category: Antibodies
• Description: PSMB4 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-QIATVTEKGVE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 25kDa band observed in lysates of cell lines NIH3T3, HEK293, HeLa, Jurkat and K562 (calculated MW of the mature protein 24.3kDa according to aa46-264 of NP_002787.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PSMB4
• UniProt ID: P28070
• NCBI: NP_002787.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PSMB4, proteasome (prosome, macropain) subunit, be
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 25kDa band observed in lysates of cell lines NIH3T3, HEK293, HeLa, Jurkat and K562 (calculated MW of the mature protein 24.3kDa according to aa46-264 of NP_002787.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248879 (0.3 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248879 (3.75 ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.