PRPF8 Antibody

• Catalog Number: orb1257215
• Category: Antibodies
• Description: PRPF8 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRPF8 (NP_006436.3).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 274kDa
• Target: PRPF8
• UniProt ID: Q6P2Q9
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: HPRP8, PRP8, PRPC8, RP13, SNRNP22pre-mRNA-processi
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PRPF8 antibody (orb1257215) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human esophagus using PRPF8 antibody (orb1257215) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using PRPF8 antibody (orb1257215) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse stomach using PRPF8 antibody (orb1257215) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U-251MG cells using PRPF8 antibody (orb1257215) at dilution of 1:100 (20x lens). Blue: DAPI for nuclear staining.