Protirelin

SKU: orb1685534

Description

Protirelin, a synthetic tripeptide analog of thyrotropin-releasing hormone (TRH), is utilized in research to stimulate the secretion of thyrotropin and prolactin. It serves as a key tool in neuroendocrinology for studying hypothalamic-pituitary-thyroid axis function in both in vitro and in vivo experimental models.

Research Area

Metabolism Research

Key Properties

• CAS Number: 24305-27-9
• MW: 362.38
• Purity: 99.76%
• Formula: C₁₆H₂₂N₆O₄
• SMILES: C([C@H](CC1=CN=CN1)NC(=O)[C@@H]2CCC(=O)N2)(=O)N3[C@H](C(N)=O)CCC3
• Target: Endogenous Metabolite,Thyroid hormone receptor(THR)
• Solubility: DMSO:67 mg/mL (184.89 mM);Ethanol:67 mg/mL (184.89 mM);H2O:66 mg/mL (182.13 mM)

Bioactivity

• Target IC50:
  THR:
• In Vivo:
  Protirelin (TRH), a neuropeptide predating the split between protostomes and deuterostomes, has likely played a role in regulating postembryonic growth and reproduction from its evolutionary origins. This study explores Protirelin's impact on body temperature, specifically through the administration of 1, 5, 10, and 20 mg/kg doses intraperitoneally to male Wistar rats (200-250 g). The findings reveal that Protirelin induces a temporary, dose-dependent increase in body temperature. Notably, thyroidectomized rats receiving a 20 mg/kg dose of Protirelin do not exhibit the significant hyperthermia observed in sham-operated counterparts, indicating that thyroid hormone release may be essential to Protirelin's hyperthermic effect.
• Animal Research:
  Protirelin (TRH tartRatse) is dissolved in physiological saline (Rats).RatsNinety male Wistar rats weighing 200-250 g are subjected to the study. In the first experiment, 50 rats are divided into five groups randomly. Four doses of Protirelin dissolved in physiological saline, i. e., 1 mg/kg, 5 mg/kg, 10 mg/kg and 20 mg/kg, are administered intraperitoneally to the four groups and saline to the remaining control group. Rectal temperature is measured at the place 5 cm inner from the anus with the electronic thermister before and after treatment with Protirelin or saline. In the second experiment, 40 rats are thyroidectomized or sham-operated under the anesthesia by thiopental sodium. Ten days after the operation, 20 mg/kg of Protirelin or saline is administered to the thyroidectomized and sham-operated animals by i. p. and rectal temperature is measured using the same method as used in the first experiment. These two experiments are undertaken from 1 p, m, to 4 p. m. and the room temperature is kept at 24±1°C through the experiments including the breeding period. For a statistical analysis, Student's t test (two-tailed) is adopted.

Storage & Handling

• Storage: keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lopremone, inhibit, EndogenousMetabolite, Endogenous Metabolite, Inhibitor, pGlu-His-Pro-NH2, Thyroidhormonereceptor(THR), Thyroliberin, Thyroidhormonereceptor, Thyroid hormone receptor(THR), Thyroid Hormone Receptor, Thyroid hormone receptor, THR, Protirelin, TRH, TRF, Synthetic thyrotropin-releasing hormone

Compound Identifiers

PubChem CID

638678