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| Catalog Number | orb766621 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to alpha-protein Kinase 1 |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ALPK1. AA range:11-60 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 139 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-ALPK1 antibody, anti-KIAA1527 antibody, anti- Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot analysis of various cells using α-protein Kinase 1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000.
Western blot analysis of lysates from COLO and HepG2 cells, using ALPK1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from 293 cells using ALPK1 antibody.
Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
