Protein C Rabbit Polyclonal Antibody

SKU: orb10109

Description

Protein C Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Vitamin K-dependent protein C heavy chain (371-460/461aa)
• Target: PROC
• Molecular Weight: 29/46 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Anticoagulant protein C; Autoprothrombin IIA; Blood coagulation factor XIV; EC 3.4.21.69; PC; PROC; PROC1; Vitamin K dependent protein C precursor; APC; EC 3.4.21.69; PC; proC; PROC_HUMAN; Protein C(inactivator of coagulation factors Va and VIIIa); Vitamin K dependent protein C; Vitamin K-dependent protein C; Anticoagulant protein C; Vitamin K-dependent protein C heavy chain.

Sample: Lane 1: Rat Kidney tissue lysates, Lane 2: Rat Colon tissue lysates, Lane 3: Rat H9C2 cell lysates, Primary: Anti-protein C (orb10109) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29/46 kDa, Observed band size: 52 kDa.

Tissue/cell: Mouse spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-protein C Polyclonal Antibody, Unconjugated (orb10109) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: Rat liver tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-protein C Polyclonal Antibody, Unconjugated (orb10109) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

PROC

UniProt

P04070