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Catalog Number | orb344229 |
---|---|
Category | Antibodies |
Description | Protein A antibody (Peroxidase) |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Isotype | IgG |
Immunogen | Protein A [Staphylococcus aureus] |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:2,000-1:20,000, IHC: 1:250, WB: 1:500-1:2,000 |
Form/Appearance | Lyophilized |
Purity | Anti-Protein A (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Protein A [Staphylococcus aureus]. Cross reactivity against Protein A from other sources is unknown. |
Conjugation | HRP |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | goat anti-Protein A Antibody, HRP Conjugated goat Read more... |
Note | For research use only |
Application notes | Anti-Protein A Peroxidase Conjugated has been tested by ELISA and western blot and is assayed against 1.0 ug of Protein A [Staphylococcus aureus] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Protein A Reduced (Lane1), Protein A Reduced (Lane2), Protein A Non-Reduced (Lane3), Protein A Non-Reduced (Lane4), Load: Lane 1 and 3 - 1.0 ug per lane, Lane 2 and 4 - 0.25 ug per lane using Protein A antibody (Peroxidase)
FC, ICC, IF, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC-Fr, IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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