Peroxiredoxin 1/PRDX1 Antibody

• Catalog Number: orb251531
• Category: Antibodies
• Description: Peroxiredoxin 1/PRDX1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Peroxiredoxin 1 (100-128aa MNIPLVSDPKRTIAQDYGVLKADEGISFR), different from the related mouse sequence by one amino acid, and identical to the related rat sequence.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunocytochemistry , 0.5-1μg/ml, Human, -Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, RatImmunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 22110 MW
• UniProt ID: Q06830
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Peroxiredoxin-1;1.11.1.15;Natural killer cell-enha
• Note: For research use only
• Application notes: WB: The detection limit for Peroxiredoxin 1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HEPG2 cells using anti-Peroxiredoxin 1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Peroxiredoxin 1 using anti-Peroxiredoxin 1 antibody.Lane 1:human Raji cell; 2:human Jurkat cell; 3:human MDA-MB-453 cell; 4:human HeLa cell; 5:human HepG2 cell; 6:human HEK293 cell; 7:human placenta tissue; 8:human A549 cell.

WB analysis of Peroxiredoxin 1 using anti-Peroxiredoxin 1 antibody.Lane 1:rat kidney tissue; 2:rat testis tissue; 3:rat liver tissue; 4:rat PC-12 cell; 5:mouse kidney tissue; 6:mouse testis tissue; 7:mouse liver tissue; 8:mouse RAW264.7 cell.

IF analysis of Peroxiredoxin 1 using anti-Peroxiredoxin 1 antibody. Peroxiredoxin 1 was detected in immunocytochemical section of HeLa cells.

IF analysis of Peroxiredoxin 1 using anti- Peroxiredoxin 1 antibody. Peroxiredoxin 1 was detected in immunocytochemical section of U20S cells.

IHC analysis of Peroxiredoxin 1 using anti-Peroxiredoxin 1 antibody. Peroxiredoxin 1 was detected in a paraffin-embedded section of Human Mammary Cancer tissue.

IHC analysis of Peroxiredoxin 1 using anti-Peroxiredoxin 1 antibody. Peroxiredoxin 1 was detected in a paraffin-embedded section of Mouse Brain tissue.

IHC analysis of Peroxiredoxin 1 using anti-Peroxiredoxin 1 antibody. Peroxiredoxin 1 was detected in a paraffin-embedded section of Rat Brain tissue.

IHC analysis of Peroxiredoxin 1 using anti-Peroxiredoxin 1 antibody.Peroxiredoxin 1 was detected in immunocytochemical section of SMMC-7721 Cell.