Prostatic Acid Phosphatase Rabbit Polyclonal Antibody

SKU: orb100732

Description

Prostatic Acid Phosphatase Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Canine, Equine, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Prostatic Acid Phosphatase (21-120/386aa)
• Target: ACP3
• Molecular Weight: 41 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ACPP; 5'-NT; ACP-3; TM-PAP; A030005E02Rik; FRAP; Lap; PAP; Ppal; Acpp11; RNACPP11; PPAP_BOVIN; ACP3; Protein tyrosine phosphatase ACP3; 3.1.3.2; PPAP_HUMAN; 5'-nucleotidase (5'-NT); Acid phosphatase 3; Ecto-5'-nucleotidase; Thiamine monophosphatase (TMPase); PPAP_MOUSE; Fluoride-resistant acid phosphatase (FRAP); PPAP_RAT;

Paraformaldehyde-fixed, paraffin embedded (Human prostate), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Prostatic Acid Phosphatase) Polyclonal Antibody, Unconjugated (orb100732) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: PC3 Cell (Human) Lysate at 40 ug, Primary: Anti-Prostatic Acid Phosphatase (orb100732) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 48 kD.

Quick Database Links

Gene Symbol

ACP3

UniProt

P15309