PROM1 Antibody

• Catalog Number: orb215910
• Category: Antibodies
• Description: PROM1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human PROM1 recombinant protein (Position: P531-H865). Human PROM1 shares 61% amino acid (aa) sequence identity with mouse PROM1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 97202 MW
• UniProt ID: O43490
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Prominin-1;Antigen AC133;Prominin-like protein 1;C
• Note: For research use only
• Application notes: WB: The detection limit for PROM1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of CACO-2 cells using anti-PROM1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of PROM1 using anti-PROM1 antibody.Lane 1:human CACO-2 cell; 2:human SW620 cell.

IHC analysis of PROM1 using anti-PROM1 antibody. PROM1 was detected in paraffin-embedded section of human liver cancer tissue.

IHC analysis of PROM1 using anti-PROM1 antibody. PROM1 was detected in paraffin-embedded section of human mammary cancer tissue.