Proinsulin Rabbit Polyclonal Antibody

• Catalog Number: orb10922
• Category: Antibodies
• Description: Proinsulin Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Equine, Human, Porcine, Rabbit
• Reactivity: Mouse, Rat
• CAS Number: 11061-68-0
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Insulin (46-59/110aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 5.8/12 kDa
• Target: INS
• UniProt ID: P01308
• RRID: AB_10753262
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: INS_HUMAN; ILPR; INS; Insulin A chain; Insulin pre
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Mouse Pancreas (C57BL/6 strain) fixed in 4% PFA for 2 hours at RT, then transferred to 30% sucrose overnight at 4°C. Treated with TBS containing 5% goat serum and 0.3% Triton X-100, to block unspecific binding sites and permeabilize cell membranes, for 30 min at RT. Antibody diluted at 1:100 in TBS containing 2% goat serum applied and incubated overnight at 4°C. Rinse sections in TBS 2x5 min on a shaker, mount section with vectorshield (with DAPI), then cover them with coverslips and seal the edge of the coverslip on the slide with nail polish.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Proinsulin) Polyclonal Antibody, Unconjugated (orb10922) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Proinsulin) Polyclonal Antibody, Unconjugated (orb10922) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Proinsulin) Polyclonal Antibody, Unconjugated (orb10922) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: rat pancreas tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Insulin Polyclonal Antibody, Unconjugated (orb10922) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated (orb868805) used at 1:200 dilution for 40 minutes at 37°C.