Prohibitin Antibody

• Catalog Number: orb1527383
• Category: Antibodies
• Description: Prohibitin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Immunogen: between 222 and 272
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Prohibitin
• UniProt ID: P35232
• NCBI: NP_002625.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: epididymis luminal protein 215; PHB1; HEL-S-54e; e
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Prohibitin by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Prohibitin (Cat. No. orb1527383) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of human Prohibitin by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Prohibitin antibody orb1527383 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.