Progesterone Receptor (phospho Ser190) Antibody [1154]

• Catalog Number: orb1273514
• Category: Antibodies
• Description: Progesterone Receptor (phospho Ser190) Antibody [1154]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1154
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Progesterone receptor (Ser190) monoclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer190 of human progesterone receptor.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 90/120
• Target: PGR
• UniProt ID: P06401
• NCBI: P06401
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: As shown in Clemm, et al. (2000), cited above, the antibody specifically reacts with the progesterone receptor phosphorylated at Ser190 in T47D cells. Applications include Immunoblotting (IB), Immunohistochemistry (IHC) and Western Blots (WB). Initial recommended range of dilutions: 1:1000 to 1:2000. When internally tested under ideal conditions the working dilutions were 1:1000 for IB, IHC and WB.
• Expiration Date: 12 months from date of receipt.

Western blot of whole cell T47D lysate prepared from cells that had been incubated in the presence of synthetic progestin agonist R5020 (500nm) showing phosphospecific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser190.