Profilin-1 Antibody

• Catalog Number: orb1264780
• Category: Antibodies
• Description: Profilin-1 Antibody
• Target: PFN1
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: This Profilin-1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 108-140 amino acids from the human region of human Profilin-1.
• UniProt ID: P07737
• MW: 15 kDa
• Tested applications: FC, WB
• Application notes: For FACS starting dilution is: 1:25For WB starting dilution is: 1:2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Profilin-1, Epididymis tissue protein Li 184a, Pro
• Note: For research use only
• NCBI: P07737

Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (OH191631) at 1/200 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Overlay histogram showing NIH/3T3 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (OH191631) at 1/200 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HUVEC whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: 293 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: C6 whole cell lysate Lysates/proteins at 20 ug per lane.