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Description
Research Area
Metabolism Research
Images & Validation
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Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. When Recombinant Human Apolipoprotein E3 is immobilized at 1μg/ml (100μl/well), the concentration of Recombinant Human VLDLR that produces 50% of the optimal binding response is found to be approximately 0. 03 - 0. 15μg/ml. |
| Target | VLDLR |
| Tag | His Tag |
| MW | 83 kDa, The protein migrates as 110-120 kDa under reducing (R) condition (SDS-Page) due to different glycosylation. |
| Purity | >97% as determined by SDS-PAGE. |
| Protein Sequence | Gly 28 - Ser 797 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−VLDLR, RP11-320E16.1, CHRMQ1, FLJ35024, VLDLRCH, VLDL receptor
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
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