TEV Protease

SKU: orb2975451

Description

TEV Protease is produced in E. coli, with a molecular weight of 54.31 kDa, and has a purity of greater than 90% as determined by SDS-PAGE.

Research Area

Protein Biochemistry

Images & Validation

• Application Notes:
  TEV Protease is a highly specific cysteine protease. The TEV Protease recognition sequence with the highest catalytic efficiency is ENLYFQ↓(S/G/A/M/C/H).

Key Properties

• Expression System: E. coli
• Endotoxins: Please contact with the lab for this information.
• Purity: >90% as determined by SDS-PAGE.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
• Form/Appearance: Liquid
• Buffer/Preservatives: 25 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM EDTA, 50% glycerol.
• Reconstitution: Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only