Calreticulin/CALR Rabbit Polyclonal Antibody

SKU: orb3116360

Description

The Calreticulin/CALR Rabbit Polyclonal Antibody (Carrier-free) is a Carrier-free antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, IHC, and WB applications. This antibody targets the Calreticulin in Human, Mouse, and Rat samples.

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Dilution Range: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human, Rat ELISA, 0.1-0.5 μg/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: E.coli-derived human Calreticulin/CALR recombinant protein (Position: T333-Q365). Human CALR shares 100% amino acid (aa) sequence identity with both mouse and rat CALR.
• Target: Calreticulin
• Molecular Weight: 50 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Carrier-free

Storage & Handling

• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CALR; Calregulin; calreticulin; cC1qR; CRP55; CRT; CRTC; ERp60; grp60; HACBP; RO; SSA

Quick Database Links

Gene Symbol

Calreticulin

UniProt

P27797