NEK7 Rabbit Polyclonal Antibody

SKU: orb3104511

Description

The NEK7 Rabbit Polyclonal Antibody (Carrier-free) is a Carrier-free antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, ICC, IHC, IP, and WB applications. This antibody targets the Serine/threonine-protein kinase Nek7 in Human, Mouse, and Rat samples.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, ICC, IHC, IP, WB
• Dilution Range: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Immunoprecipitation, 2-4 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human NEK7 recombinant protein (Position: M1-S302). Human NEK7 shares 97.7% and 98% amino acid (aa) sequence identity with mouse and rat NEK7, respectively.
• Target: Serine/threonine-protein kinase Nek7
• Molecular Weight: 35 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Carrier-free

Storage & Handling

• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NEK7; NimA related protein kinase 7

Quick Database Links

Gene Symbol

Serine/threonine-protein kinase Nek7

UniProt

Q8TDX7