RNase A (DNase free)

SKU: orb532708

Description

RNase A is an endoribonuclease that attacks at the 3'-phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with ssRNA. RNase A is free of detectable DNase and protease activity, a heat treatment of the enzyme is not necessary before use. Stability: RNase A is an extremely stable enzyme, remarkable resistant to heating. It renatures easily after treatment with most denaturing agents.

Images & Validation

• Application Notes:
  Applications: 1) Plasmid and genomic DNA preparation 2) Removal of RNA from recombinant protein preparations 3) Ribonuclease protection assays 4) Mapping single-base mutations in DNA or RNA.

Key Properties

• Biological Activity: ≥ 80 Kunitz units/mg
• CAS Number: 9001-99-4
• MW: Theoretical MW: 13.7 kDa (monomer)
• Purity: ≥ 90% (ion exchange chromatography), salt free, chromatographically homogeneous lyophilisate

Storage & Handling

• Storage: store at 2-8 °C
• Form/Appearance: dry powder
• Concentration: Working concentration: 1-100 µg/ml (depending on application). The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase cleaves ss and dsRNA as well as the RNA strand in RNA-DNA hybrids.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Endoribonuclease, Bovine pancreas