RecombinantI-TAC/CXCL11,Human

SKU: orb1494810

Description

I-TAC (Interferon-inducible T-cell α Chemoattractant), also known as CXCL11, is a chemokine belonging to the CXCL subfamily. Along with Interferon-γ induced Protein 10 kDa and Monokine Induced by Interferon-γ, I-TAC is strongly up-regulated by Interferon-γ and binds to the G-protein coupled receptor CXCR3. The expression of CXCR3 is associated with Th1-mediated immune responses and mediates chemotaxis of Th1 cells. I-TAC induces, maintains, and amplifies the inflammatory reactions, and sustains chronic immune responses against self-antigens. Although CXCR3 was thought to be the exclusive receptor of I-TAC initially, more evidence has shown that I-TAC can also bind to CXCR4 and CXCR7, and that I-TAC plays important roles in tumor development and angiostasis. Recombinant human I-TAC/CXCL11 (rhI-TAC) produced in E.coli is a single non-glycosylated polypeptide chain containing 74 amino acids. A fully biologically active molecule, rhI-TAC has a molecular mass of 8.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Research Area

Neuroscience; Immunology & Inflammation

Images & Validation

• Application Notes:
  Reconstituted in ddH2O at 100 μg/mL.

Key Properties

• Source: Escherichia coli.
• Biological Activity: ED50 400 units/mg.
• Purification: > 95% as analyzed by SDS-PAGE and HPLC.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• MW: 8.4 kDa, observed by reducing SDS-PAGE.
• Purity: > 95% as analyzed by SDS-PAGE and HPLC.
• Protein Sequence: MFPMFKRGR CLCIGPGVKA VKVADIEKAS IMYPSNNCDK IEVIITLKEN KGQRCLNPKS KQARLIIKKV ERKNF

Storage & Handling

• Storage: Lyophilized recombinant human I-TAC/CXCL11 (rhI-TAC) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhI-TAC remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Form/Appearance: Lyophilized after extensive dialysis against PBS.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ITAC, CXCL11