Recombinant Mouse IL-4RA Protein

SKU: orb2994779

Description

Recombinant Mouse Interleukin-4 Receptor Subunit Alpha is produced by our Mammalian expression system and the target gene encoding Ile26-Arg233 is expressed with a human IgG1 Fc tag at the C-terminus.

Research Area

Immunology & Inflammation

Key Properties

• Expression System: Human Cells
• Biological Origin: Mouse
• Biological Activity: ELISA: Immobilized Mouse IL-4RA-Fc(Cat#orb2994779) at 2μg/ml (100 μl/well)can bind Mouse IL-4-His(Cat#orb3002476) .The ED50 of Mouse IL-4-His(Cat#orb3002476)is 4.32 ng/ml.. BLI: Loaded Mouse IL-4-His(Cat#orb3002476) on HIS1K Biosensor, can bind Mouse IL-4RA-Fc(Cat#orb2994779) with an affinity constant of -3 nM as determined in BLI assay.
• Tag: C-Fc
• Expression Region: Ile26-Arg233
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 51.5 KDa. Observed: 65-80 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interleukin-4 receptor subunit alpha;IL-4R-alpha;CD124;IL4-BP;Soluble IL-4R-alpha

Quick Database Links

UniProt

P16382