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Description
Research Area
Immunology & Inflammation
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Mouse |
| Biological Activity | BLI: Loaded Anti-Human IL23-P19 mAb (Cat#orb2993116) on Pro-A Biosensor, can bind Recombinant Mouse IL-23 (Cat#orb2994202) with an affinity constant of 1.57 uM as determined in BLI assay. (Regularly tested). Cell Based Assay: Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells.The ED50 for this effect is 501.08 ng/ml. |
| Tag | Tag free |
| Expression Region | Val22-Ala196&Met23-Ser335 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 19.7&35.8 KDa. Observed: 18&40-55 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 90% as determined by SEC-HPLC. (QC verified) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SGRF; IL-23p19; CLMF p40; IL-12 subunit p40; NKSF2
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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