Recombinant Human uPAR Protein

SKU: orb2995110

Description

Recombinant Human Urokinase Plasminogen Activator Surface Receptor is produced by our Mammalian expression system and the target gene encoding Leu23-Arg303 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human uPAR-His(Cat#orb2995110) at 2μg/ml (100 μl/well) can bind Human uPA-His*(Cat#orb2993548).*: Biotinylated by NHS-biotin prior to testing. The ED50 of Human uPA-His(Cat#orb2993548) is 7.19 ng/ml. (Regularly tested) Immobilized Recombinant Human uPAR (C-6His)(Cat#orb2995110) at 2μg/ml (100 μl/well) can bind Anti-Human uPAR Antibody(Mouse IgG1).The ED50 of Anti-Human uPAR Antibody(Mouse IgG1) is 5.26ng/ml.(Regularly tested)
• Tag: C-6His
• Expression Region: Leu23-Arg303
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 32.4 KDa. Observed: 52 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM Tris-HCl, 10% Trehalose, 2% Mannitol, 0.05% Tween 80, pH 8.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Urokinase Plasminogen Activator Surface Receptor; U-PAR; uPAR; Monocyte activation antigen Mo3; CD87; PLAUR; MO3; UPAR

Quick Database Links

UniProt

Q03405