Human SEM1 Recombinant Protein (N-His-SUMO & C-Strep)

SKU: orb2963935

Description

Recombinant Human SEM1 Protein, N-His-SUMO & C-Strepexpressed in E. coli , spanning Ser2-Ser70. Purity: >90% by SDS-PAGE. Highlights His-Tagged: N-terminal 6×His tag for IMAC purification. E. coli Expression: High-yield, cost-effective production. High Purity: >90% purity verified by SDS-PAGE.

Images & Validation

• Tested Applications: ELISA, SDS-PAGE, WB

Key Properties

• Expression System: E. coli
• Biological Origin: Homo sapiens (Human)
• Expression Region: Ser2-Ser70
• MW: 21.77 kDa
• Purity: >90% as determined by SDS-PAGE.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
• Reconstitution: Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Split hand/foot malformation type 1 protein, Deleted in split hand/split foot protein 1, DSS1, SHFDG1, SHFM1, Split hand/foot deleted protein 1, C7orf76, 26S proteasome complex subunit SEM1, SEM1, 26S proteasome complex subunit DSS1

Quick Database Links

UniProt

P60896