Recombinant Human RANK L Protein

SKU: orb2994417

Description

Recombinant Human Receptor Activator of NF-kappa-B Ligand is produced by our E.coli expression system and the target gene encoding Ile140-Asp317 is expressed.

Research Area

Immunology & Inflammation

Key Properties

• Expression System: E.coli
• Biological Origin: Human
• Biological Activity: BLI: Loaded Human RANK-His(Cat#orb2994416) on HIS1K Biosensor, can bind Human RANK L (Cat#orb2994417) with an affinity constant of <1 pM as determined in BLI assay. (Regularly tested) Loaded Recombinant Human OPG-Fc (Cat#orb3002306) on Pro A Biosensor, can bind Human RANKL (Cat#orb2994417) with an affinity constant of 1.83 pM as determined in BLI assay.
• Tag: Tag free
• Expression Region: Ile140-Asp317
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 22.4 KDa. Observed: 20 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD254; ODF; OPGL; RANK L; TNFSF11; CD254; Osteoclast differentiation factor; Receptor activator of nuclear factor kappa-B ligand; tumor necrosis factor ligand superfamily member 11

Quick Database Links

UniProt

O14788