Recombinant Human MICA Protein

SKU: orb2994621

Description

Recombinant Human MHC Class I Polypeptide-Related Sequence A is produced by our Mammalian expression system and the target gene encoding Glu24-Gln308 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Mouse NKG2D (N-6His)(Cat. No.:orb2993855) at 2 μg/ml (100 μl/well) can bind Human MICA (C-Fc)(Cat. No.:orb2994621). The EC50 of Human MICA (C-Fc)(Cat. No.:orb2994621) is not higher than 10 ng/ml.
• Tag: C-Fc
• Expression Region: Glu24-Gln308
• Endotoxins: < 0.01 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 60 KDa. Observed: 85-110 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MHC Class I Polypeptide-Related Sequence A; MIC-A; MICA; PERB11.1

Quick Database Links

UniProt

AAH16929.1