Recombinant Human IL-6RA Protein

SKU: orb2993733

Description

Recombinant Human Interleukin-6 receptor subunit alpha is produced by our Mammalian expression system and the target gene encoding Leu20-Pro365 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Anti-Human IL6R Antibody (SAR_bio, Research Grade)(Cat#NC373) at 1μg/ml (100 μl/well) can bind Recombinant Human IL -6RA (C-Fc)(Cat#orb2993733)*. *: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Human IL -6RA (C-Fc)(Cat#orb2993733) is 1.78 ng/ml.(Regularly tested). BLI: Loaded Recombinant Rhesus Macaque IL-6 (C-6His) (Cat#orb2992743) on HIS1K Biosensor, can bind Recombinant Human IL-6RA (C-Fc) (Cat#orb2993733) with an affinity constant of 21.6 nM as determined in BLI assay. (Regularly tested)
• Tag: C-Fc
• Expression Region: Leu20-Pro365
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 65.4 KDa. Observed: 85-100 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interleukin-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6R 1; Membrane glycoprotein 80; gp80; CD126

Quick Database Links

UniProt

P08887