Recombinant Human IgG1 Fc Protein

SKU: orb2993960

Description

Recombinant Human Immunoglobulin G1 Fc is produced by our Mammalian expression system and the target gene encoding Asp104-Lys330(Asp239Glu,Leu241Met) is expressed.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human IgG1 Fc (Cat#orb2993960) at 2 μg/ml (100 μl/well) can bind Recombinant Human CD64 (C-6His) (Cat#orb2994349). The ED50 of Recombinant Human CD64 (C-6His)(Cat#orb2994349) is 30-150 ng/ml .. BLI: Loaded Human IgG1(Cat#orb2993960) on Protein A Biosensor, can bind Human CD64-His(Cat#orb2994349) with an affinity constant of 0.11 nM as determined in BLI assay.
• Tag: Tag free
• Expression Region: Asp104-Lys330(Asp239Glu,Leu241Met)
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 25.9 KDa. Observed: 32 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

g gamma-1 chain C region; IGHG1

Quick Database Links

UniProt

P01857