Recombinant Human GPNMB Protein

SKU: orb2995035

Description

Recombinant Human Glycoprotein Non-Metastatic Melanoma Protein B is produced by our Mammalian expression system and the target gene encoding Lys23-Pro486 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human GPNMB (C-6His)(Cat#orb2995035) at 2μg/ml (100 μl/well) can bind Anti-Human GPNMB Antibody (GLE_bio, Research Grade)(Cat#orb2992498).The ED50 of Anti-Human GPNMB Antibody (GLE_bio, Research Grade)(Cat#orb2992498) can bind is 10.84 ng/ml. (Regularly tested).
• Tag: C-6His
• Expression Region: Lys23-Pro486
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 53.09 KDa. Observed: 80-120 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Transmembrane Glycoprotein NMB; Transmembrane Glycoprotein HGFIN; GPNMB; HGFIN; NMB

Quick Database Links

UniProt

Q14956