Recombinant Human GPC3 Protein

SKU: orb2993899

Description

Recombinant Human Glypican-3 is produced by our Mammalian expression system and the target gene encoding Gln25-His559 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: BLI: Loaded Human FGFb (157AA) (Cat#orb3002481) on APS Biosensor, can bind Human GPC3-Fc(Cat#orb2993899) with an affinity constant of 0.7 nM as determined in BLI assay. (Regularly tested) Loaded Human FGFb (155AA) (Cat#orb3002364) on APS Biosensor, can bind Human GPC3-Fc(Cat#orb2993899) with an affinity constant of 0.68 nM as determined in BLI assay. (Regularly tested) Loaded Human FGFb (146AA) (Cat#orb3002475) on APS Biosensor, can bind Human GPC3-Fc(Cat#orb2993899) with an affinity constant of 0.6 nM as determined in BLI assay. (Regularly tested)
• Tag: C-Fc
• Expression Region: Gln25-His559
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 87.7 KDa. Observed: 40 KDa & 65 KDa & 90-250 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Glypican-3; GTR2-2; Intestinal protein OCI-5; MXR7; GPC3; OCI5

Quick Database Links

UniProt

P51654