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Description
Research Area
Metabolism Research
Images & Validation
−
| Tested Applications | ELISA, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Species/Host | E. coli |
|---|---|
| Tag | His-tag |
| MW | 30.41 kDa |
| Purity | >90% as determined by SDS-PAGE. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
|---|---|
| Buffer/Preservatives | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−25-hydroxyvitamin D(3) 1-alpha-hydroxylase, CYP27B, CYP27B1, Cytochrome P450C1 alpha, Calcidiol 1-monooxygenase, Cytochrome P450VD1-alpha, Cytochrome p450 27B1, CYP1ALPHA, Cytochrome P450 subfamily XXVIIB polypeptide 1, 25-OHD-1 alpha-hydroxylase, VD3 1A hydroxylase, 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
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Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis