DENV-4 Envelope protein E Recombinant Protein (N-His)

SKU: orb2966258

Description

Recombinant DENV-4 Envelope protein E Protein, N-His is produced in E. coli and supplied in liquid form. The protein includes the regions of Met576-Lys679 and carries the N-His tag. With a molecular weight of 13.46 kDa, it is suitable for ELISA, SDS-PAGE and WB applications. This product has a purity of greater than 90% as determined by SDS-PAGE.

Images & Validation

• Tested Applications: ELISA, SDS-PAGE, WB

Key Properties

• Expression System: E. coli
• Biological Origin: Dengue virus 4 (DENV-4)
• Expression Region: Met576-Lys679
• MW: 13.46 kDa
• Purity: >90% as determined by SDS-PAGE.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Reconstitution: Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Genome polyprotein, Capsid protein C, Core protein, Protein prM, Peptide pr, Small envelope protein M, Matrix protein, Envelope protein E, Non-structural protein 1, NS1, Non-structural protein 2A, NS2A, Serine protease subunit NS2B, Flavivirin protease NS2B regulatory subunit, Non-structural protein 2B, Serine protease NS3, 3.4.21.91, 3.6.1.15, 3.6.4.13, Flavivirin protease NS3 catalytic subunit, Non-structural protein 3, Non-structural protein 4A, NS4A, Peptide 2k, Non-structural protein 4B, NS4B, RNA-directed RNA polymerase NS5, 2.1.1.56, 2.1.1.57, 2.7.7.48, Non-structural protein 5

Quick Database Links

UniProt

Q2YHF0