Recombinant Cynomolgus TIGIT Protein

SKU: orb2994063

Description

Recombinant Cynomolgus T cell Immunoreceptor With Ig And ITIM Domains is produced by our Mammalian expression system and the target gene encoding Met89-Pro209 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Cynomolgus
• Biological Activity: ELISA: Immobilized Human Cynomolgus TIGIT-Fc(Cat#orb2994063) at 10μg/ml (100 μl/well) can bind Rhesus Macaque CD155-His(Cat#orb2994061).The ED50 of Rhesus Macaque CD155-His(Cat#orb2994061)is 0.66 ug/ml.. BLI: Loaded Human PVR-His(Cat#orb2993514) on HIS1K Biosensor, can bind Cynomolgus TIGIT-Fc(Cat#orb2994063) with an affinity constant of 24.6 nM as determined in BLI assay.
• Tag: C-Fc
• Expression Region: Met89-Pro209
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 40.6 KDa. Observed: 45-55 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

T-cell immunoreceptor with Ig and ITIM domains; VSIG9; VSTM3; TIGIT; V-set and transmembrane domain-containing protein 3; V-set and immunoglobulin domain-containing protein 9

Quick Database Links

UniProt

G7NXM4