Tnfsf11 Antibody

SKU: orb345227

Description

RANK-L antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:10,000, WB: 1:1000
• Reactivity: Mouse
• Application Notes:
  This purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19-20 kDa in size corresponding to the mature mouse RANKL protein by western blotting in appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse RANKL protein (aa 143-316) produced in E.coli.
• Target: Tnfsf11
• Purity: Anti-RANK L antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse RANKL protein. A BLAST analysis was used to suggest cross-reactivity with RANKL from mouse sources based on 100% homology with the immunizing sequence. Based on 97% homology, cross-reactivity with rat, 91% with Chinese hamster, 90% with Thirteen-lined ground squirrel; other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-RANK-L Antibody, Receptor activator of nuclear factor kappa B, TNF-related activation-induced cytokine, TRANCE, Tumor necrosis factor ligand superfamily member 11, TNF11, TNFRSF11, Osteoprotegerin ligand, OPG, Osteoclast differentiation factor, ODF, CD254

Quick Database Links

Gene Symbol

Tnfsf11

UniProt

O35235

RefSeq (Protein):NP_035743.2