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Description
Images & Validation
−| Tested Applications | IHC, IP |
|---|---|
| Dilution Range | WB - Not recommended. orb1527564 has not performed satisfactorily when used for WB of NELFE in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of NELFE.; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Predicted Reactivity | Bovine |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 330 and C-terminus |
| Target | NELFE |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−D6S45; major histocompatibility complex gene RD; negative elongation factor E; negative elongation factor polypeptide E; NELF-E; nuclear protein; RD; RD RNA-binding protein; RDBP; RDP; RNA-binding protein RD
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002895.3 |
|---|
Protocol Information
IHC
Immunohistochemistry
IP
Immunoprecipitation












