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PD-L1/CD274 Rabbit Polyclonal Antibody

SKU: orb3078670

Description

The PD-L1/CD274/PD Rabbit Polyclonal Antibody (Carrier-free) is a Carrier-free antibody raised in rabbit. It is immunogen affinity purified and suitable for use in FC and WB applications. This antibody targets the Programmed cell death 1 ligand 1 in Human samples.

Research Area

Cancer Biology, Disease Biomarkers, Immunology & Inflammation

Images & Validation

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Tested ApplicationsFC, WB
Dilution RangeWestern blot, 0.25-0.5μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ReactivityHuman
Application Notes
For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

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Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenE.coli-derived human PD-L1 recombinant protein (Position: E45-T290). Human PD-L1 shares 69% amino acid (aa) sequence identity with mouse PD-L1.
TargetProgrammed cell death 1 ligand 1
Molecular Weight40-50 kDa
PurificationImmunogen affinity purified.
ConjugationCarrier-free

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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B7 H; B7 H1; B7 homolog 1; B7H1; CD274; CD274 molecule; PD L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PD-L1/CD274; Programmed death ligand 1

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

UniProt Details

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No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol

Available Sizes

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100 μg
$ 470.00
Free Secondary Antibody (20 ul)0/0

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