PI3 Kinase p85/p55 (phospho-Tyr467/199) Rabbit Polyclonal Antibody

SKU: orb14998

Description

This is a rabbit polyclonal antibody targeting PI3 Kinase p85/p55. It is unconjugated and suitable for use in IF, WB applications. The antibody is reactive with human, mouse, rat samples. Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500~1:1000; IF: 1:100-1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide-KLH. Peptide sequence around phosphorylation site of tyrosine 467 (L-Y(p)-E-E-Y) derived from Human PI3 Kinase p85/p55
• Target: PI3 Kinase p85/p55
• Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

p85, AGM7, p85-ALPHA, p55, p55-GAMMA, PIK3R1

Quick Database Links

Gene Symbol

PI3 Kinase p85/p55

UniProt

P27986

Gang Yang 1, Fei Su 2, Bin-Xiao Han 3, Hong-Xin Su 4, Chen-Hao Guo 2, Shao-Hua Yu 2, Quan-Lin Guan 5, Xiao-Ming Hou HNF1A induces glioblastoma by upregulating EPS8 and activating PI3K/AKT signaling pathway Biochem Pharmacol, (2024)

PubMed: 38494066

Applications

WB

Reactivity

Human

Zhang, Yuquan et al. Circ_0030235 knockdown protects H9c2 cells against OGD/R-induced injury via regulation of miR-526b PeerJ, 9, e11482 (2021)

PubMed: 34820154