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Description
Research Area
Cancer Biology, Signal Transduction
Images & Validation
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| Tested Applications | ELISA, ICC, IF, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000 ELISA: 1/10000 |
| Reactivity | Human, Mouse, Porcine, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Smad2/3 around the phosphorylation site of Thr8. |
| Target | SMAD2/SMAD3 |
| Molecular Weight | Calculated MW: 52 kDa; Observed MW: 48-52，60 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SMAD2; MADH2; MADR2; MAD homolog 2; M hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2;SMAD3; MADH3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3;smad2/3;smad2+3
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Available Sizes
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