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Description
Research Area
Images & Validation
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Key Properties
−| Source | CHO cells |
|---|---|
| Biological Activity | Biological activity was determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 cells using a concentration of IC50 0.5-0.7 μg/mL. Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
| Target | SPARC |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses. |
| Protein Sequence | APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI |
Storage & Handling
−| Storage | The lyophilized Osteonectin recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted Osteonectin stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing Osteonectin recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human SPARC Protein [orb2995106]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 33.73 KDa. Observed: 36 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman Secreted Acidic and Rich in Cysteine Recombinant Protein [orb1905953]
> 98 % by SDS-PAGE and HPLC analyses.
Approximately 32.7 kDa, a single non-glycosylated polypeptide chain containing 286 amino acids.
500 μg, 10 μg, 100 μgHuman SPARC Protein, hFc Tag [orb1290920]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 58.8 kDa after removal of the signal peptide. The apparent molecular mass of hFc-SPARC is approximately 55-70 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μgHuman Secreted Acidic and Rich in Cysteine Recombinant Protein, His [orb1905954]
> 95 % by SDS-PAGE and HPLC analyses.
Approximately 36.1 kDa, a single non-glycosylated polypeptide chain containing 314 amino acids, with expression vector sequence (containing 6x His tag).
10 μg, 100 μg, 500 μgSPARC/Osteonectin Rabbit Polyclonal Antibody [orb2951577]
ELISA, IHC, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
50 μg, 100 μg

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Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_003109.1 |
|---|



