NRF1/NRF-1 Antibody

SKU: orb1538076

Description

NRF1/NRF-1 Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IHC, IHC-P, WB
• Dilution Range: IHC, IHC-P (10 µg/ml), WB (0.2 - 1 µg/ml)
• Reactivity: Human, Mouse
• Application Notes:
  Does not react with: Mouse. Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic peptide located between aa360-409 of human NRF1 (Q16656, NP_005002). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (100%).
• Target: NRF1 / NRF-1
• Purification: Immunoaffinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.09% sodium azide, 2% sucrose
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NRF1, EWG, NRF-1, Nuclear respiratory factor 1, ALPHA-PAL

Quick Database Links

Gene Symbol

NRF1 / NRF-1