NR5A2/LRH1 Antibody

SKU: orb1248278

Description

NR5A2/LRH1 Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human, Porcine
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in nuclear lysates of cell lines HeLa and HepG2, and approx 60kDa band observed in Pig Ovary lysates (calculated MW of 56.5kDa according to Human NP_003813.1 and Pig AFD98842.1). Recommended concentration: 0.3-1µg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Liver and NIH-3T3 cell line for this particular batch.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-TDYDRSPFVTSPIS
• Target: NR5A2 / LRH1
• Molecular Weight: Approx 55kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NR5A2, nuclear receptor subfamily 5, group A, member 2, B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2, CYP7A promoter-binding factor, alpha-1-fetoprotein transcription factor, b1-binding factor, hepatocyte transcription factor which activates enhancer II of hepatitis B virus, fetoprotein-alpha 1 (AFP) transcription factor, hepatocytic transcription factor, liver nuclear receptor homolog-1 variant 2, liver receptor homolog 1, liver receptor homolog-1, nuclear receptor NR5A2, nuclear receptor subfamily 5 group A member 2

Quick Database Links

Gene Symbol

NR5A2 / LRH1

UniProt

O60587

RefSeq (Protein):NP_995582.1, NP_003813.1