Nicastrin, C-terminal Antibody

SKU: orb3167669

Description

Our Anti-Nicastrin, C-terminal rabbit polyclonal primary antibody detects human Nicastrin, C-terminal, and is IgG. It is validated for use in ICC, WB.

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB: 3-10 µg/mL
• Reactivity: Human
• Application Notes:
  Human or mouse brain samples commonly prepared with reducing (50 mM DTT), urea (2.3 M), SDS (1.5%) in 62.5 mM Tris-HCL pH 6.8 sample buffer (without boiling) heating to 50 C for 15 min. Unprocessed full length human Nicastrin is 709 amino acids, however this protein contains an N-terminal signal peptide which is considered to undergo cleavage during processing and transit to the cell plasma membrane, in addition the protein undergoes glycosylation to produce a glycoprotein of about 145 kDa apparent MW by SDS PAGE.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide (C-NAKADVLFIAPREPGAVSY) corresponding to human Nicastrin [691-709] in the C-terminal region conjugated via additional N-terminal Cys to Diphtheria toxoid.
• Target: Nicastrin, C-terminal
• Molecular Weight: 145 kDa (See application details.)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized from PBS, pH 7.4. Contains no preservative.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Nicastrin, C-terminal

UniProt

Q92542