NFkB-p65 (Phospho-Ser536) Rabbit Polyclonal Antibody

SKU: orb14753

Description

This is a rabbit polyclonal antibody targeting NFkB-p65. It is unconjugated and suitable for use in IF, IHC, IP, WB applications. The antibody is reactive with human, monkey, mouse, rat samples. Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Dilution Range: IF: 1:50-200; WB: 1:500 - 1:2000.; IHC: 1:100 - 1:300.; IP: 2-5 ug:mg lysate.
• Reactivity: Human, Monkey, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide-KLH. Peptide sequence around phosphorylation site of serine 536 (F-S-S(p)-I-A) derived from Human NFkB-p65
• Target: NFkB-p65
• Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide.
• Concentration: 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NFKB3; RELA; TF65; Transcription factor p65; p65

Quick Database Links

Gene Symbol

NFkB-p65

UniProt

Q04206

C-H Wang, P-J Wang, Y-C Hsieh, S Lo, Y-C Lee, Y-C Chen, C-H Tsai, W-C Chiu, S Chu-Sung Hu, C-W Lu, Y-F Yang, C-C Chiu, F Ou-Yang, Y-M Wang, M-F Hou, S-SF Yuan Resistin facilitates breast cancer progression via TLR4-mediated induction of mesenchymal phenotypes and stemness properties Oncogene, 37, 589 (2017)

PubMed: 28991224

Applications

IHC

Reactivity

Human

Chen Li, Su Pan, Yan Song, Yinqing Li, Ji Qu Silence of lncRNA MIAT protects ATDC5 cells against lipopolysaccharides challenge via up-regulating miR-132 Artificial Cells Nanomedicine and Biotechnology, 47, 2521 (2019)

PubMed: 31204523

Applications

WB

Reactivity

Mouse

Yang, Xifeng et al. Downregulation of miR‑181b inhibits human colon cancer cell proliferation by targeting CYLD and inhibiting the NF‑κB signaling pathway Int J Mol Med, 46, 1755-1764 (2020)

PubMed: 32901872